Journal: bioRxiv

Article Title: GBA1 deficiency differentially affects endolysosomal trafficking in neurons versus astrocytes

doi: 10.64898/2026.01.12.697563

Figure Lengend Snippet: (A) Diagram of iPSC cell line generation and differentiation protocol into midbrain dopaminergic neurons and astrocytes, with r epresentative images of immunocytochemistry of GBA1 +/+ iPSC-neurons stained with anti-TH (green) and DAPI and GBA1 +/+ iPSC-astrocytes stained with anti-S100β (red) and DAPI (B) Quantification of glucocerebrosidase (GCase) enzyme activity by relative fluorescence of 4-methylumbelliferyl-β-D-glucopyranoside (4-MUG) in indicated genotypes. GCase activity of all cell lines treated with CBE was subtracted to eliminate background activity. (one-way ANOVA F (4, 19) =9.092, p=0.0003). (C) Representative Western blot with anti-GBA1 protein in GBA1 +/+ , GBA1 IVS/+ , GBA1 +/+ + conduritol B epoxide (CBE), and GBA1 IVS/IVS iPSC-neurons. (D) Quantification of GBA1 protein in neurons from 3 independent replicates in indicated genotypes, normalized to Actin in control (one-way ANOVA F (3,8) =108.6, p<0.0001). (E) Heatmap of differentially expressed midbrain dopaminergic neuron-and astrocyte-specific genes in GBA1 +/+ neurons versus GBA1 +/+ astrocytes sorted by z-score. Statistical significance determined by one-way ANOVA (ns = p ≥ 0.05, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001). Data are presented as mean ± SEM.

Article Snippet: After 2 weeks in Astrocyte Maturation Medium, a small portion of cells was fixed and stained to evaluate the presence of mature astrocyte markers: mouse anti-glial fibrillary acidic protein (GFAP) (Sigma-Aldrich G3893, 1:400), mouse anti-Vimentin (Invitrogen #MA5-11883, 1:500), rabbit anti-Vimentin (Cell Signaling Technology 5741S, 1:100), rabbit anti-S100β (Proteintech 15146-1-AP, 1:100), and rabbit anti-Aquaporin 4 (AQP4Invitrogen #PA5-85767, 1:100).

Techniques: Immunocytochemistry, Staining, Activity Assay, Fluorescence, Western Blot, Control

Journal: Life

Article Title: Silicon Dioxide Nanoparticles Alter Social Behavior, Color Preference, Oxidative Stress Markers, and Histological Structure of Brain Regions in Zebrafish ( Danio rerio )

doi: 10.3390/life15111715

Figure Lengend Snippet: Appearance of the rostro-caudal axis of the telencephalon in zebrafish from control and experimental groups: HE, PCNA, GFAP and S100 stain. Scale bar: 50 μm. Arrowheads indicate immunopositive cells.

Article Snippet: Primary antibodies were applied overnight at 4 °C in a humidified atmosphere as follows: rabbit polyclonal anti-GFAP antibody (1:1000 dilution, cat. no. 173002, Synaptic Systems, Goettingen, Germany), rabbit monoclonal anti-S100β antibody (1:1000 dilution, cat. no. C48942 , Signalway Antibody), and rabbit polyclonal anti-PCNA antibody (1:250 dilution, cat. no. PA5-27214, Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Control, Staining

Journal: Life

Article Title: Silicon Dioxide Nanoparticles Alter Social Behavior, Color Preference, Oxidative Stress Markers, and Histological Structure of Brain Regions in Zebrafish ( Danio rerio )

doi: 10.3390/life15111715

Figure Lengend Snippet: Quantification of glial fibrillary acidic protein (GFAP) ( A ), proliferating cell nuclear antigen (PCNA) ( B ), and S100 calcium-binding protein B (S100B) ( C ) cells in the telencephalon of adult zebrafish after exposure to SiO 2 NPs. Data are expressed as mean ± SEM. ** p < 0.01.

Article Snippet: Primary antibodies were applied overnight at 4 °C in a humidified atmosphere as follows: rabbit polyclonal anti-GFAP antibody (1:1000 dilution, cat. no. 173002, Synaptic Systems, Goettingen, Germany), rabbit monoclonal anti-S100β antibody (1:1000 dilution, cat. no. C48942 , Signalway Antibody), and rabbit polyclonal anti-PCNA antibody (1:250 dilution, cat. no. PA5-27214, Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Binding Assay

Journal: Life

Article Title: Silicon Dioxide Nanoparticles Alter Social Behavior, Color Preference, Oxidative Stress Markers, and Histological Structure of Brain Regions in Zebrafish ( Danio rerio )

doi: 10.3390/life15111715

Figure Lengend Snippet: Histology of adult zebrafish brain from control and experimental groups. The untreated adult zebrafish brain shows intact structural morphology and cellular morphology. The 4 layers of the optic tectum: (I) superficial gray-white zone, (II) central zone, (III) deep white zone, and (IV) periventricular gray zone (PGZ). The lateral torus (TLa) is homogenous, diffusively arranged, with no apparent divisions or inflammation. The brain section has undamaged cellular morphology which represents non-existent inflammation. Rows show IHC staining for anti-GFAP, anti-PCNA, and anti-S100. First row shows hematoxylin-eosin (HE) staining. Scale bar: 50 μm. Rows show IHC staining for anti-GFAP, anti-PCNA and anti-S100. First row shows hematoxylin-eosin (HE) staining. Scale bar: 50 μm. Arrowheads indicate immunopositive cells. Symbols: *—edema area (green); *—neuronal rarefaction (black).

Article Snippet: Primary antibodies were applied overnight at 4 °C in a humidified atmosphere as follows: rabbit polyclonal anti-GFAP antibody (1:1000 dilution, cat. no. 173002, Synaptic Systems, Goettingen, Germany), rabbit monoclonal anti-S100β antibody (1:1000 dilution, cat. no. C48942 , Signalway Antibody), and rabbit polyclonal anti-PCNA antibody (1:250 dilution, cat. no. PA5-27214, Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Control, Immunohistochemistry, Staining

Journal: Life

Article Title: Silicon Dioxide Nanoparticles Alter Social Behavior, Color Preference, Oxidative Stress Markers, and Histological Structure of Brain Regions in Zebrafish ( Danio rerio )

doi: 10.3390/life15111715

Figure Lengend Snippet: Quantification of glial fibrillary acidic protein (GFAP) ( A ), proliferating cell nuclear antigen (PCNA) ( B ), and S100 calcium-binding protein B (S100B) ( C ) cells in the optic tectum of adult zebrafish after exposure to SiO 2 NPs. Data are expressed as mean ± SEM. * p < 0.05; ** p < 0.01.

Article Snippet: Primary antibodies were applied overnight at 4 °C in a humidified atmosphere as follows: rabbit polyclonal anti-GFAP antibody (1:1000 dilution, cat. no. 173002, Synaptic Systems, Goettingen, Germany), rabbit monoclonal anti-S100β antibody (1:1000 dilution, cat. no. C48942 , Signalway Antibody), and rabbit polyclonal anti-PCNA antibody (1:250 dilution, cat. no. PA5-27214, Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Binding Assay

Journal: Cell Reports

Article Title: Multi-site investigation of gut microbiota in CDKL5 deficiency disorder mouse models: Targeting dysbiosis to improve neurological outcomes

doi: 10.1016/j.celrep.2025.115546

Figure Lengend Snippet:

Article Snippet: Cortical sections were incubated for 2 h in a blocking solution containing 10% BSA (w/vol) and 0.3% Triton X-100 (vol/vol) in PBS and incubated overnight at 4°C with rabbit polyclonal anti-S100β primary antibody (GeneTex, Cat. no. GTX129573; RRID: AB_2886037 ) diluted 1:250, in PBS with 5% BSA (w/vol) and 0.15% Triton X-100 (vol/vol).

Techniques: Recombinant, SYBR Green Assay, Plasmid Preparation, Reverse Transcription, Mutagenesis, Software, Imaging, Spectrophotometry, Real-time Polymerase Chain Reaction, Microscopy